Keywords
Antibiogram profile
Cheese whey
Antibacterial activity.
Article Number
024Abstract
Diarrhoeal diseases remain a major public health problem and are among the leading causes of morbidity and mortality in children, particularly in developing countries. Among the bacterial pathogens associated with childhood diarrhoea, Escherichia coli is frequently implicated, with diarrhoeagenic strains contributing significantly to gastrointestinal infections. Although the infection can be treated with antibiotics however the emergence of multi antibiotic-resistant E. coli has complicated treatment therefore the need for alternative antibacterial therapies. This study therefore evaluates cheese whey for antibacterial activity against diarrhoeagenic E. coli isolated from stool samples of diarrhoeic children in the bid of searching for alternative therapies for treating bacterial diarrhoea caused by E. coli. The influence of types of coagulants in whey production on its antibacterial properties was also studied. A total of 55 stool samples was collected from diarrhoeal children at selected primary health centers and government hospitals in Owo, Ondo State, Nigeria. The collected stools were analyzed using standard microbiological, biochemical and molecular techniques to identify the types of bacteria present. The antibiogram profile of the isolated E. coli was determined using standard microbiological assays. The antibacterial activity of commercially prepared whey (CPW) was compared with that of the laboratory prepared whey (LPW) against the isolated E. coli using agar well diffusion assay. So also the effect of types of coagulants used in the production of whey on its antibacterial activity using standard methods. E. coli was isolated in 47.3% of the stool samples examined. Other bacterial species isolated are Staphylococcus aureus (21.8%), Pseudomonas aeruginosa (16.4%), and Salmonella enterica (14.6%). The antibiogram profile of the isolated E. coli revealed high levels of multi-antibiotic resistance (MAR) index (0.4-1.0) among the isolates to pefloxacin, amoxicillin, augmentin and streptomycin but were susceptible to ciprofloxacin. CPW demonstrated antibacterial activity against some of the isolated E. coli whereas LPW exhibited broader and stronger antibacterial activity on all the test E. coli isolates except one. The inhibition mediated by LPW was superior to that mediated by ciprofloxacin on 7 (26.9%) of the E. coli isolates and was at per with the inhibition mediated by ciprofloxacin on most of the E. coli isolates. Whey prepared using Calotropis procera as coagulant showed the highest growth inhibitory activity compared with whey prepared using lime and alum. The findings demonstrate that cheese whey possesses antibacterial properties against the isolated diarrhoeagenic E. coli, with efficacy influenced by coagulant type. Therefore, cheese whey can be explored in the development of a more effective antibacterial drug that can be exploited in the management of diarrhoea caused by E. coli especially in the absence of ciprofloxacin which is the most effective antibiotic against the isolated diarrhoeagenic E. coli.References
Adebolu, T.T. and Babafemi, E.O. (2008). Epidermiological survey of the most prevalent bacteria responsible for infantile diarrhoea in South west, Nigeria. Journal of Pure and Applied Microbiology, 2(1):69-72.
Adebolu, T. T., Olorunfemi, O. B. (2016). Effects of fermented cheese whey on the cells of the immune system of apparently healthy albino rats. Journal of Immunobiology1(2), 1-2.
Amenu, D., & Bacha, K. (2025). Bio-preservation potential and antimicrobial activity of bacteriocin-producing lactic acid bacteria isolated from Ethiopian traditional fermented dairy products. Probiotics and Antimicrobial Proteins, 17(5), 3402-3415.
Anwar, A., Faiz, M. A., Badar, I. H., Jaspal, M. H., & Hou, J. (2025). Influence of fermentation time and storage conditions on the physicochemical properties of different yogurt varieties using starter cultures and probiotic Lactobacillus rhamnosus GG. Processes, 13(3), 759.
Ashraf, A., Sabu, S., Sasidharan, A., & Sunooj, K. V. (2025). Natural feed supplements from crustacean processing side streams for improved growth of finfishes and crustaceans: a review. Journal of Animal Physiology and Animal Nutrition, 109(2), 376-401.
Behera, D. K., & Mishra, S. (2022). The burden of diarrhea, etiologies, and risk factors in India from 1990 to 2019: evidence from the global burden of disease study. BMC public health, 22(1), 92.
Biswas, S., Bal, M., Pati, S., Rana, R., Dixit, S., & Ranjit, M. (2024). Antibiotic resistance in toxigenic E. coli: a severe threat to global health. Discover Medicine, 1(1), 72.
Cheesbrough, M. (2006). District Laboratory Practice in tropical Countries (2nd ed.). Cambridge University Press.
Chen, X., Bai, H., Mo, W., Zheng, X., Chen, H., Yin, Y., & Peng, H. (2025). Lactic acid bacteria bacteriocins: safe and effective antimicrobial agents. International Journal of Molecular Sciences, 26(9), 4124.
Das, R., Palit, P., Haque, M. A., Ahmed, T., & Faruque, A. S. G. (2022). Association between pathogenic variants of diarrheagenic Escherichia coli and growth in children under 5 years of age in the Global Enteric Multicenter Study. The American journal of tropical medicine and hygiene, 107(1), 72.
Del Carpio, A. M., Freire, C. A., Gomes, T. A., Abe, C. M., & Elias, W. P. (2025). Importance of aggR sequence variants detection for accurate molecular diagnosis of enteroaggregative Escherichia coli. Microbiology Spectrum, 13(11), e01441-25.
Dela, H., Egyir, B., Majekodunmi, A. O., Behene, E., Yeboah, C., Ackah, D., & Addo, K. K. (2022). Diarrhoeagenic E. coli occurrence and antimicrobial resistance of Extended Spectrum Beta-Lactamases isolated from diarrhoea patients attending health facilities in Accra, Ghana. PLoS One, 17(5), e0268991.
Dereje, B., Yibabie, S., Keno, Z., & Megersa, A. (2023). Antibiotic utilization pattern in treatment of acute diarrheal diseases: the case of Hiwot Fana Specialized University Hospital, Harar, Ethiopia. Journal of Pharmaceutical Policy and Practice, 16(1), 62.
El Alami El Hassani, N., Baraket, A., & Alem, C. (2025). Recent advances in natural food preservatives: A sustainable solution for food safety and shelf-life extension. Journal of Food Measurement and Characterization, 19(1), 293-315.
El-Garhi, H. E. M., Abd-Elghany, A. A., Ibrahim, A. M., El-Aidie, S. A., & Castro-Muñoz, R. (2025). Effect of organic acids on pathogenic and lactic acid bacteria in directly raw milk cheddar cheese. Probiotics and Antimicrobial Proteins, 1-21.
Eugster, E., Jakob, E., & Wechsler, D. (2012). Cheese, processed cheese, and whey. In Ullmann's Encyclopedia of Industrial Chemistry. Wiley-VCH Verlag GmbH & Co. KGaA. https://doi.org/10.1002/14356007.a06_163.pub2
Fakhkhari, P., Tajeddin, E., Azimirad, M., Salmanzadeh-Ahrabi, S., Abdi-Ali, A., Nikmanesh, B., & Alebouyeh, M. (2022). Involvement of Pseudomonas aeruginosa in the occurrence of community and hospital acquired diarrhea, and its virulence diversity among the stool and the environmental samples. International Journal of Environmental Health Research, 32(1), 61-71.
Hasan, R. N., Jasim, S. A., & Ali, Y. H. (2022). Detection of fimH, kpsMTII, hlyA, and traT genes in Escherichia coli isolated from Iraqi patients with cystitis. Gene Reports, 26, 101468.
Hetsa, B.A., Yeboah, E.E.A., Ismail, A. Essack S.Y., Amoako D.G. (2026). Pathogenomics of multidrug-resistant Escherichia coli from bloodstream infections in South Africa. BMC Med Genomics 19, 71 (2026). https://doi.org/10.1186/s12920-026-02349-y
Jaaz, W. S., & Kadham, E. J. (2026). Identification of virulence factors genies (eaeA) and (bpfA) of Enteropathogenic Escherichia coli strains isolated from children with diarrhea in Kerbala city. Perinatal Journal, 34(1), 96-102.
Kumawat, M., & Sharma, M. (2025). Virulence Gene Profiles of Diarrheagenic Escherichia coli: A Comparative Analysis of Pathotypes Isolated from Acute Diarrheal Patients. International Journal of Research in Medical and Basic Sciences (IJRMBS), 11(10), 1-14.
Luparelli, A., Trisciuzzi, D., Schirinzi, W. M., Caputo, L., Smiriglia, L., Quintieri, L., & Monaci, L. (2025). Whey proteins and bioactive peptides: Advances in production, selection and bioactivity profiling. Biomedicines, 13(6), 1311.
Ma, Y., Sun, H., Zhang, S., Yang, C., Musazade, E., Fan, H., & Zhang, Y. (2025). Structural modification of whey protein isolate via electrostatic complexation with Tremella polysaccharides and its effect on emulsion stability at pH 4.5. International Journal of Biological Macromolecules, 297, 139870.
Makarova, M. A. (2023). A modern view of diarrheagenic Escherichia coli—a causative agent of acute intestinal infections. Journal of microbiology, epidemiology and immunobiology, 100(4), 333-344.
Malos, I. G., Ghizdareanu, A. I., Vidu, L., Matei, C. B., & Pasarin, D. (2025). The role of whey in functional microorganism growth and metabolite generation: A biotechnological perspective. Foods, 14(9), 1488.
Manhique-Coutinho, L., Chiani, P., Michelacci, V., Taviani, E., Bauhofer, A. F. L., Chissaque, A., & De Deus, N. (2022). Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea: A cross-sectional study in four provinces of Mozambique: Diarrheagenic Escherichia coli in Mozambique. International Journal of Infectious Diseases, 121, 190-194.
Medugu, N., Aworh, M. K., Iregbu, K., Nwajiobi-Princewill, P., Abdulraheem, K., Hull, D. M., & Thakur, S. (2022). Molecular characterization of multi drug resistant Escherichia coli isolates at a tertiary hospital in Abuja, Nigeria. Scientific reports, 12(1), 14822.
Murshed, M., AL-Tamimi, J., Mares, M., Aljawdah, H. M., & Al-Qurishy, S. (2025). Effectiveness of Calotropis procera Leaf Extract in Treating Eimeriosis-Induced Inflammation Alteration of the Jejunum in Mice. Journal of the Hellenic Veterinary Medical Society, 76(2), 9085-9096.
Okebuagwu, Q.C., Adebolu, T.T. and Ojo, B.A. (2017). Types of bacteria associated with diarrhoeal cases among children in Akure, Nigeria and their antibiogram profile. Journal of Advances in Microbiology, 2(2):1-10
Olayinka, A. A., Oginni-Falajiki, I. O., Okeke, I. N., & Aboderin, A. O. (2024). Diarrhoeagenic Escherichia coli associated with childhood diarrhoea in Osun state, Nigeria. BMC Infectious Diseases, 24(1), 928.
Onelli, R. R. V., de Jesus, J. C., Lins, R. P., da Capela, A. P., Soares, L. S., & Ferrão, S. P. B. (2026). Technological innovation in the production of matured buffalo cheese using artichoke flower extract (Cynara cardunculus L. var. scolymus) as a coagulant: organic acid profile, proteomic and sensory study. International Dairy Journal, 173, 106491.
Sakr, S. S., Alharbi, T. A., & Hassan, M. F. (2026). Research Article Functional Evaluation of Moringa Seed Extracts as Plant-Based Coagulants for Camel Milk. Int. J. Dairy Sci, 21, 14-23.
Sangchi, Z. G., Peyravi, M., & Jahanshahi, M. (2026). A Comparative Study of Bio-Based Antimicrobial Membranes Induced by Medicinal Plant-Derived Biocide for Dairy Wastewater Treatment. Journal of Polymers and the Environment, 34(3), 51.
Saxena, S., Khetra, Y., Ganguly, S., & Sasmal, S. (2025). Morphological and Textural Properties of Feta Cheese Made of Vegetable Protease. Food Bioengineering, 4(2), 142-154.
Taviani, E., & Pedro, O. (2022). Impact of the aquatic pathobiome in low-income and middle-income countries (LMICs) quest for safe water and sanitation practices. Current opinion in biotechnology, 73, 220-224.
Tilahun, M., Belete, M. A., Gedefie, A., Debash, H., Alemayehu, E., Weldehana, D. G., & Shibabaw, A. (2025). Prevalence of Salmonella and Shigella species and their multidrug resistance patterns among pediatric populations in Ethiopia: a systematic review and meta-analysis. BMC Infectious Diseases, 25(1), 52.
Tille, P.M. (2017). Bailey and Scoutt’s Diagnostic Microbiology (14th ed.). Elsevier.
Viana, G. G. F., Cardozo, M. V., Pereira, J. G., & Rossi, G. A. M. (2025). Antimicrobial resistant Staphylococcus spp., Escherichia coli, and Salmonella spp. in food handlers: a global review of persistence, transmission, and mitigation challenges. Pathogens, 14(5), 496.
Vildan Akdeniz1 , Melisa Ünal1 , Nayil Dinkç (2023). The Importance and Health benefits of Whey Proteins. Journal of Hygienic Engineering and Design. Review paper UDC 637.344.045:613.292
World Health Organisation (WHO). (2024). Diarrhoeal Diseases. https://www.who.int/news-room/fact-sheets/detail/diarrhoeal-disease
Xu, J., Hei, X., Zheng, M., Wang, Y., Xie, N., Li, X., & Zhang, S. (2025). Effect of heat-resistant protease from psychrotrophic bacteria in raw milk on shelf-life quality of UHT milk. Food Control, 111927.
Zelelie, T. Z., Eguale, T., Yitayew, B., Abeje, D., Alemu, A., Seman, A., & Abebe, T. (2023). Molecular epidemiology and antimicrobial susceptibility of diarrheagenic Escherichia coli isolated from children under age five with and without diarrhea in Central Ethiopia. Plos one, 18(7), e0288517.
Zulfkar, Q., Humaira, A., Mohd, A. D., & Afshana, Q. (2025). The growing threat of antibiotic resistance: Mechanisms, causes, consequences, and solutions. International Journal of Cognitive Neuroscience and Psychology, 3(3), 28-36.
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Copyright (c) 2026 Komolafe Tolulope Omolola, Adebolu T. T, Daniels, A. O (Author)