Keywords
Antibiogram Profile
Multiple Antibiotic Resistance Index
Article Number
048Abstract
Klebsiella pneumoniae is a pathogen that is of a great health concern globally. This is primarily due to its rapidly increasing antimicrobial resistance (AMR) profile. K. pneumoniae is responsible for a wide range of life-threatening infections, including pneumonia, urinary tract infections, bloodstream infections, and sepsis particularly among immunocompromised and hospitalized patients. The clinical management of infections caused by this organism has become increasingly difficult owing to its ability to evade the effects of multiple antimicrobial agents. This study therefore is to determine the occurrence rate of this organism in clinical samples collected from patients attending selected hospitals in the community sampled and also assess the antibiogram profile of this organism to update statistics. A total of 120 clinical samples (urine, sputum, wound swabs, stool) was collected from patients attending selected government hospitals in Akure, Nigeria. Antibiotic susceptibility testing was performed using Kirby-Bauer disc diffusion assay and Multiple Antibiotic Resistance Index (MARI) calculated. A total of 206 bacterial isolates were recovered from the 120 clinical samples evaluated of which 105 (87.5%) are identified as K. pneumoniae. The highest incidence of K. pneumoniae was observed in sputum (37.4%), followed by 31.3% in urine while the least (10.9%) was observed in wound swabs. Majority of the isolated K. pneumoniae were resistant to cefuroxime, ceftriaxone, cephorex, ciprofloxacin, ceftazidime, augmentin with MARI values ranging from 0.2 to 1.0 confirming extensive multidrug resistance.References
Abegunrin, R. O., Amisu, K. O., Al-Huchaimi, S. N., Ajoseh, S. O., Salami, W. O., Lawal-Sanni, A. O., Saki, M., & Akinyemi, K. O. (2026). Evaluation of occurrence, antimicrobial resistance rates, and antibiotic resistance determinants in Klebsiella pneumoniae from hospitalised patients in Lagos, Nigeria. BMC Microbiology, 26, 471.
Al-Dhabaan, F. A. (2019). Morphological, biochemical and molecular identification of petroleum hydrocarbons biodegradation bacteria isolated from oil polluted soil in Dhahran, Saud Arabia. Saudi Journal of Biological Sciences, 26(6), 1247-1252.
Anyanwu, M. U., and Okoye, R. C. (2017). Antimicrobial activity of Nigerian medicinal plants. Journal of intercultural Ethnopharmacology, 6(2), 240.
Braun, H. G., Perera, S. R., Tremblay, Y. D., and Thomassin, J. L. (2024). Antimicrobial resistance in Klebsiella pneumoniae: an overview of common mechanisms and a current Canadian perspective. Canadian Journal of Microbiology, 70(12), 507-528.
Cireșă, A., Tălăpan, D., Vasile, C. C., Popescu, C., and Popescu, G. A. (2024). Evolution of antimicrobial resistance in Klebsiella pneumoniae over 3 years (2019–2021) in a tertiary hospital in Bucharest, Romania. Antibiotics, 13(5), 431.
Clinical and Laboratory Standards Institute (CLSI). (2023). Performance standards for antimicrobial susceptibility testing (33rd ed.). CLSI supplement M100.
Clinical and Laboratory Standards Institute (CLSI). (2025). Performance standards for antimicrobial susceptibility testing (35th ed.). CLSI supplement M100.
Dadgostar, P. (2019). Antimicrobial resistance: implications and costs. Infection and drug resistance, 3903-3910.
Das, A., Behera, B. K., Acharya, S., Paria, P., Chakraborty, H. J., Parida, P. K., and Das, B. K. (2019). Genetic diversity and multiple antibiotic resistance index study of bacterial pathogen, Klebsiella pneumoniae strains isolated from diseased Indian major carps. Folia Microbiologica, 64(6), 875-887.
Ding, J., Yan, W., Zheng, R., Ma, M., and Jiang, L. (2025). Combating Klebsiella pneumoniae: from antimicrobial resistance mechanisms to phage-based combination therapies. Frontiers in Cellular and Infection Microbiology, 15, 1691215.
Gehring, R., Mochel, J. P., and Schmerold, I. (2023). Understanding the background and clinical significance of the WHO, WOAH, and EMA classifications of antimicrobials to mitigate antimicrobial resistance. Frontiers in Veterinary Science, 10, 1153048.
Giubelan, L., Dragonu, L., Stoian, A. C., and Dumitrescu, F. (2021). Antibiotic resistance profile of Klebsiella pneumoniae strains isolated in an infectious diseases clinic. Romanian J Infect Dis, 24(1), 32-37.
Khan, Z. A., Siddiqui, M. F., and Park, S. (2019). Current and emerging methods of antibiotic susceptibility testing. Diagnostics, 9(2), 49.
Mudenda, S., Wataya, M. D., Mufwambi, W., and Chizimu, J. Y. (2024). The World Health Organization Access, Watch, and Reserve classification of antibiotics: an awareness survey among pharmacy professionals in a sub-Saharan country, Zambia. Antimicrobial Stewardship & Healthcare Epidemiology, 4(1), e212.
Nazir, A., Nazir, A., Zuhair, V., Aman, S., Sadiq, S. U. R., Hasan, A. H., ... and Bulimbe, D. B. (2025). The global challenge of antimicrobial resistance: mechanisms, case studies, and mitigation approaches. Health Science Reports, 8(7), e71077.
Ogefere, H. O., & Idoko, M. O. (2024). Multiple antibiotic resistance index of Klebsiella pneumoniae isolated from clinical specimens in a tertiary hospital in Benin City, Nigeria. Journal of Medicine and Biomedical Research, 23(1), 5–11. https://doi.org/10.4314/jmbr.v23i1.3
Onyeji, C. B., Enitan, S. S., Kemiki, O. A., Igwe, A. C., Adeniyi, A. A., Iduh, M. U., Itodo, G. E., Okuneye, A. O., Adamson, P. O., & Kolawole, M. F. (2024). Molecular detection of OXA-48 and NDM-1 carbapenemase genes among clinical isolates of Klebsiella pneumoniae recovered from patients attending a private tertiary hospital in Southwestern Nigeria. BMC Infectious Diseases, 24, 970. https://doi.org/10.1186/s12879-024-09869-x
Osundiya, O. O., Oladele, R. O., and Oduyebo, O. O. (2013). Multiple antibiotic resistance (MAR) indices of Pseudomonas and Klebsiella species isolates in Lagos University Teaching Hospital. African Journal of Clinical and Experimental Microbiology, 14(3), 164-168.
Paczosa, M. K., and Mecsas, J. (2016). Klebsiella pneumoniae: going on the offense with a strong defense. Microbiology and Molecular Biology Reviews, 80(3), 629-661.
Paneri, M., and Sevta, P. (2023). Overview of antimicrobial resistance: An emerging silent pandemic. Global Journal of Medical Pharmaceutical and Biomedical Update, 18, 1.
Shah, A. A., Alwashmi, A. S., Abalkhail, A., and Alkahtani, A. M. (2025). Emerging challenges in Klebsiella pneumoniae: Antimicrobial resistance and novel approach. Microbial Pathogenesis, 202, 107399.
Syal, K., Mo, M., Yu, H., Iriya, R., Jing, W., Guodong, S., ... and Tao, N. (2017). Current and emerging techniques for antibiotic susceptibility tests. Theranostics, 7(7), 1795.
Wang, L., Li, Y., Zhang, J., Xiao, H., and Xia, C. (2026). Investigating risk factors and antibiotic resistance of bloodstream infections causing Klebsiella pneumoniae in burn patients. Medicine, 105(2), e47037.
World Health Organization. (2024). WHO bacterial priority pathogens list, 2024: Bacterial pathogens of public health importance to guide research, development and strategies to prevent and control antimicrobial resistance. World Health Organization.
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