Keywords
Enteric Bacteria
Hand-Dug Wells
Microbial Quality
Nigeria
Sabon Gida
Abstract
In many semi-urban Nigerian communities like Sabon Gida, hand-dug wells are primary domestic water sources. However, poor sanitation and unregulated antibiotic use often compromise their safety. This study evaluated the microbial quality of water and antibiotic susceptibility profiles of enteric bacteria isolated from ten (10) hand-dug wells in Sabon Gida. Using standard microbiological techniques, Total Viable Counts (TVC), Total Coliform (TCC), and Faecal Coliform Counts (FCC) were determined. Isolates were characterized via biochemical testing, and susceptibility was assessed using the Kirby-Bauer disc diffusion method. All wells exceeded WHO and Nigerian drinking water standards. TVC ranged from 1.6 x 105 CFU/mL to 9.3 x 105 CFU/mL, while faecal coliforms (12 CFU/100mL–37 CFU/100mL) violated the zero-tolerance (0 CFU/100mL) safety threshold. Of the 23 isolates recovered, Escherichia coli was most prevalent (52.2%), followed by Klebsiella pneumoniae (34.8%) and Enterobacter aerogenes (13.0%). Susceptibility testing showed 100% resistance to Ampicillin and Tetracycline, and 91.3% to Amoxicillin/Clavulanate. Conversely, all isolates were sensitive to Gentamicin and Chloramphenicol. The presence of multi-drug resistant (MDR) enteric bacteria indicates significant faecal contamination and a high potential for waterborne disease transmission. Urgent community-wide water treatment, improved well construction, and stricter antibiotic stewardship are required to mitigate these public health risks.
References
1. Adetunde, I. A., & Glover, R. L. (2010). Bacteriological quality of borehole water, well water and sachet water in Kwaso, Ashanti Region, Ghana. Journal of American Science, 6(9), 180–184.
2. Adzitey, F. (2020). Antibiotic resistance of Escherichia coli isolated from beef and its environment in Techiman Municipality, Ghana. Journal of Applied Sciences and Environmental Management, 24(7), 1153–1157. https://doi.org/10.4314/jasem.v24i7.7
3. Amaechi, N. M. (2015). Bacteriological quality of some hand-dug wells in Abia State, Nigeria. Nigerian Journal of Microbiology, 29, 3112–3119.
4. American Public Health Association (APHA). (2017). Standard methods for the examination of water and wastewater (23rd ed.). APHA.
5. Ashbolt, N. J. (2015). Microbial contamination of drinking water and disease outcomes in developing regions. Toxicology, 198(1-3), 229–238. https://doi.org/10.1016/j.tox.2004.01.030
6. Brian, F., & Catalina, A. P. (2015). Bacteriological examination of waters: Membrane filtration protocol. American Society for Microbiology (ASM).
7. Bulus, L., Ishaya, S., & Yohanna, W. (2021). Impact of pit latrine proximity on the microbial quality of hand-dug wells in rural communities. African Journal of Environmental Science, 15(2), 88–102.
8. Cheesbrough, M. (2010). Medical laboratory manual for tropical countries (pp. 40–45). Butterworth Limited.
9. Clinical and Laboratory Standards Institute (CLSI). (2023). Performance standards for antimicrobial susceptibility testing (33rd ed.). CLSI supplement M100.
10. Gambo, J. B., James, Y., & Yakubu, M. B. (2015). Physico-chemical and bacteriological analysis of well water at Crescent Road Poly Quarters, Kaduna. International Journal of Engineering and Science, 4(11), 11–17.
11. Idowu, A. O., Oluremi, B. B., & Odubawo, K. M. (2011). Bacteriological analysis of well water samples in Sagamu. African Journal of Clinical and Experimental Microbiology, 12(2), 86–91.
12. Igharo, I. E., Osadolor, H. B., & Odijie, E. C. (2021). Antibiotic susceptibility patterns of enteric bacteria isolated from borehole water in selected communities. African Journal of Health Sciences, 21(2), 45–52.
13. Malik, A., Yasar, A., Tabinda, A., & Abubakar, M. (2012). Water-borne diseases, cost of illness and willingness to pay for diseases interventions in rural communities of developing countries. Iranian Journal of Public Health, 41(6), 39–49.
14. McFadyen, S. (2014). Drinking water: Assessing and managing risk. Water Quality Research Journal of Canada, 49(1), 3–4.
15. Ngwa, N. R., & Chrysanthus, N. (2013). Bacteriological analysis of well water sources in the Bambui student residential area. Journal of Water Resource and Protection, 5(1), 1013–1017.
16. Nkere, C. K., Ibe, N. I., & Iroegbu, C. U. (2011). Bacteriological quality of foods and water sold by vendors and in restaurants in Nsukka, Enugu State, Nigeria: A comparative study of three microbiological methods. Journal of Health, Population and Nutrition, 29(6), 560–566. https://doi.org/10.3329/jhpn.v29i6.9891
17. Nyandjou, Y. M. C., Yakubu, S. E., Abdullahi, I. O., & Machido, D. A. (2017). Screening for multidrug resistant Escherichia coli O157:H7 isolated from refuse dumpsites in Zaria metropolis, Nigeria. Nigerian Journal of Science Research, 16(3), 276–281.
18. Ocheri, C., Mile, I. I., & Anyam, R. W. (2017). Assessment of water quality of hand dug wells in some selected settlements in Nigeria. Journal of Environmental Science, Toxicology and Food Technology, 11(10), 57–63. https://doi.org/10.9790/2402-1110015763
19. Odonkor, S. T., & Addo, K. K. (2018a). Bacteria resistance to antibiotics: Recent reports from Ghana. International Journal of Microbiology, 2018, Article ID 202508.
20. Odonkor, S. T., & Addo, K. K. (2018b). Prevalence of multi-drug-resistant Escherichia coli isolated from drinking water sources. International Journal of Microbiology, 2018, Article 7204013. https://doi.org/10.1155/2018/7204013
21. Okonko, I. O., Ogunleye, V. O., Adeniji, F. O., & Babalola, E. T. (2022). Microbiological and physicochemical analysis of different water sources in selected communities: A comparative study of groundwater quality. Nature and Science, 20(1), 12–25.
22. Olowe, O. A., Idris, O. J., & Taiwo, S. S. (2022). Prevalence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in environmental water sources in Nigeria. Environmental Monitoring and Assessment, 194(4), 280. https://doi.org/10.1007/s10661-022-09934-x
23. Standards Organisation of Nigeria (SON). (2015). Nigerian standard for drinking water quality (NIS 554:2015). SON.
24. World Bank. (2023). The silent burden: Economic impacts of waterborne diseases and antimicrobial resistance. World Bank Publications.
25. World Health Organization (WHO). (2022). Guidelines for drinking-water quality: Fourth edition incorporating the first and second addenda. WHO.
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